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Sophion Bioscience qpatch automated patch-clamp platform
Na v channel compound profile assessed on the Qube or <t>Qpatch*.</t> Ten-point titrations in the Qube automated patch-clamp assay determined MSD199 to be a potent and selective compound (IC 50 = 4.7 ± 0.8 nM, mean ± SD). MSD199 potency was too weak to calculate for all other Na v isoforms except Na v 1.4, which was 1781-fold shifted to 8370 nM mean IC 50 ± 1429.
Qpatch Automated Patch Clamp Platform, supplied by Sophion Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+patch+clamp+qpatch/qpatch+16+automated+electrophysiology+platform/pmc11808707-32-55-59
Average 90 stars, based on 1 article reviews
qpatch automated patch-clamp platform - by Bioz Stars, 2026-11
90/100 stars

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1) Product Images from "Analgesia and peripheral c-fiber modulation by selective Na v 1.8 inhibition in rhesus"

Article Title: Analgesia and peripheral c-fiber modulation by selective Na v 1.8 inhibition in rhesus

Journal: Pain

doi: 10.1097/j.pain.0000000000003404

Na v channel compound profile assessed on the Qube or Qpatch*. Ten-point titrations in the Qube automated patch-clamp assay determined MSD199 to be a potent and selective compound (IC 50 = 4.7 ± 0.8 nM, mean ± SD). MSD199 potency was too weak to calculate for all other Na v isoforms except Na v 1.4, which was 1781-fold shifted to 8370 nM mean IC 50 ± 1429.
Figure Legend Snippet: Na v channel compound profile assessed on the Qube or Qpatch*. Ten-point titrations in the Qube automated patch-clamp assay determined MSD199 to be a potent and selective compound (IC 50 = 4.7 ± 0.8 nM, mean ± SD). MSD199 potency was too weak to calculate for all other Na v isoforms except Na v 1.4, which was 1781-fold shifted to 8370 nM mean IC 50 ± 1429.

Techniques Used: Patch Clamp

Related Articles

Patch Clamp:

Article Title: Termination of Vernakalant-Resistant Atrial Fibrillation by Inhibition of Small-Conductance Ca 2+ -Activated K + Channels in Pigs
Article Snippet: In Vitro Electrophysiology Effects of AP14145 on hSK3 (KCa2.3) and hERG (hKV11.1) were investigated by automated patch clamp (Qpatch; Biolin Scientific, Sophion, Denmark) as previously described.19 Likewise, the effect on rNaV1.5 was evaluated as earlier described20 but with a modified voltage protocol so that rNaV1.5 currents were elicited with 1-second interval by a 50-ms depolarizing step to −20 mV from a holding potential of −120 mV (a total of 80 pulses).

Article Title: Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.
Article Snippet: Many spider-venom peptides are known to modulate the activity of the voltage-gated sodium (NaV) subtype 1.7 (NaV1.7) channel, which has emerged as a promising analgesic target.. In particular, a class of spider-venom peptides (NaSpTx1) has been found to potently inhibit NaV1.7 (nanomolar IC50), and has been shown to produce analgesic effects in animals.. However, one member of this family [m-TRTX-Hhn2b (Hhn2b)] does not inhibit mammalian NaV channels expressed in dorsal root ganglia at concentrations up to 100 mM.

Article Title: Reply to: How Many SARS-CoV-2 "Viroporins" Are Really Ion Channels?
Article Snippet: Using an automated patch clamp technique with gigaseal formation and Rs compensation (QPatch, Sophion, Denmark), we have in preliminary experiments compared induced vs. non-induced HEK293 cells stably expressing ORF10 under the control of tetracycline.

Stable Transfection:

Article Title: Termination of Vernakalant-Resistant Atrial Fibrillation by Inhibition of Small-Conductance Ca 2+ -Activated K + Channels in Pigs
Article Snippet: In Vitro Electrophysiology Effects of AP14145 on hSK3 (KCa2.3) and hERG (hKV11.1) were investigated by automated patch clamp (Qpatch; Biolin Scientific, Sophion, Denmark) as previously described.19 Likewise, the effect on rNaV1.5 was evaluated as earlier described20 but with a modified voltage protocol so that rNaV1.5 currents were elicited with 1-second interval by a 50-ms depolarizing step to −20 mV from a holding potential of −120 mV (a total of 80 pulses).

Article Title: Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.
Article Snippet: Many spider-venom peptides are known to modulate the activity of the voltage-gated sodium (NaV) subtype 1.7 (NaV1.7) channel, which has emerged as a promising analgesic target.. In particular, a class of spider-venom peptides (NaSpTx1) has been found to potently inhibit NaV1.7 (nanomolar IC50), and has been shown to produce analgesic effects in animals.. However, one member of this family [m-TRTX-Hhn2b (Hhn2b)] does not inhibit mammalian NaV channels expressed in dorsal root ganglia at concentrations up to 100 mM.

Article Title: Reply to: How Many SARS-CoV-2 "Viroporins" Are Really Ion Channels?
Article Snippet: Using an automated patch clamp technique with gigaseal formation and Rs compensation (QPatch, Sophion, Denmark), we have in preliminary experiments compared induced vs. non-induced HEK293 cells stably expressing ORF10 under the control of tetracycline.

Expressing:

Article Title: Termination of Vernakalant-Resistant Atrial Fibrillation by Inhibition of Small-Conductance Ca 2+ -Activated K + Channels in Pigs
Article Snippet: In Vitro Electrophysiology Effects of AP14145 on hSK3 (KCa2.3) and hERG (hKV11.1) were investigated by automated patch clamp (Qpatch; Biolin Scientific, Sophion, Denmark) as previously described.19 Likewise, the effect on rNaV1.5 was evaluated as earlier described20 but with a modified voltage protocol so that rNaV1.5 currents were elicited with 1-second interval by a 50-ms depolarizing step to −20 mV from a holding potential of −120 mV (a total of 80 pulses).

Article Title: Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.
Article Snippet: Many spider-venom peptides are known to modulate the activity of the voltage-gated sodium (NaV) subtype 1.7 (NaV1.7) channel, which has emerged as a promising analgesic target.. In particular, a class of spider-venom peptides (NaSpTx1) has been found to potently inhibit NaV1.7 (nanomolar IC50), and has been shown to produce analgesic effects in animals.. However, one member of this family [m-TRTX-Hhn2b (Hhn2b)] does not inhibit mammalian NaV channels expressed in dorsal root ganglia at concentrations up to 100 mM.

Article Title: Reply to: How Many SARS-CoV-2 "Viroporins" Are Really Ion Channels?
Article Snippet: Using an automated patch clamp technique with gigaseal formation and Rs compensation (QPatch, Sophion, Denmark), we have in preliminary experiments compared induced vs. non-induced HEK293 cells stably expressing ORF10 under the control of tetracycline.

Control:

Article Title: Termination of Vernakalant-Resistant Atrial Fibrillation by Inhibition of Small-Conductance Ca 2+ -Activated K + Channels in Pigs
Article Snippet: In Vitro Electrophysiology Effects of AP14145 on hSK3 (KCa2.3) and hERG (hKV11.1) were investigated by automated patch clamp (Qpatch; Biolin Scientific, Sophion, Denmark) as previously described.19 Likewise, the effect on rNaV1.5 was evaluated as earlier described20 but with a modified voltage protocol so that rNaV1.5 currents were elicited with 1-second interval by a 50-ms depolarizing step to −20 mV from a holding potential of −120 mV (a total of 80 pulses).

Article Title: Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.
Article Snippet: Many spider-venom peptides are known to modulate the activity of the voltage-gated sodium (NaV) subtype 1.7 (NaV1.7) channel, which has emerged as a promising analgesic target.. In particular, a class of spider-venom peptides (NaSpTx1) has been found to potently inhibit NaV1.7 (nanomolar IC50), and has been shown to produce analgesic effects in animals.. However, one member of this family [m-TRTX-Hhn2b (Hhn2b)] does not inhibit mammalian NaV channels expressed in dorsal root ganglia at concentrations up to 100 mM.

Article Title: Reply to: How Many SARS-CoV-2 "Viroporins" Are Really Ion Channels?
Article Snippet: Using an automated patch clamp technique with gigaseal formation and Rs compensation (QPatch, Sophion, Denmark), we have in preliminary experiments compared induced vs. non-induced HEK293 cells stably expressing ORF10 under the control of tetracycline.

In Vitro:

Article Title: Termination of Vernakalant-Resistant Atrial Fibrillation by Inhibition of Small-Conductance Ca 2+ -Activated K + Channels in Pigs
Article Snippet: In Vitro Electrophysiology Effects of AP14145 on hSK3 (KCa2.3) and hERG (hKV11.1) were investigated by automated patch clamp (Qpatch; Biolin Scientific, Sophion, Denmark) as previously described.19 Likewise, the effect on rNaV1.5 was evaluated as earlier described20 but with a modified voltage protocol so that rNaV1.5 currents were elicited with 1-second interval by a 50-ms depolarizing step to −20 mV from a holding potential of −120 mV (a total of 80 pulses).

Article Title: Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.
Article Snippet: Many spider-venom peptides are known to modulate the activity of the voltage-gated sodium (NaV) subtype 1.7 (NaV1.7) channel, which has emerged as a promising analgesic target.. In particular, a class of spider-venom peptides (NaSpTx1) has been found to potently inhibit NaV1.7 (nanomolar IC50), and has been shown to produce analgesic effects in animals.. However, one member of this family [m-TRTX-Hhn2b (Hhn2b)] does not inhibit mammalian NaV channels expressed in dorsal root ganglia at concentrations up to 100 mM.

Article Title: Reply to: How Many SARS-CoV-2 "Viroporins" Are Really Ion Channels?
Article Snippet: Using an automated patch clamp technique with gigaseal formation and Rs compensation (QPatch, Sophion, Denmark), we have in preliminary experiments compared induced vs. non-induced HEK293 cells stably expressing ORF10 under the control of tetracycline.

Modification:

Article Title: Termination of Vernakalant-Resistant Atrial Fibrillation by Inhibition of Small-Conductance Ca 2+ -Activated K + Channels in Pigs
Article Snippet: In Vitro Electrophysiology Effects of AP14145 on hSK3 (KCa2.3) and hERG (hKV11.1) were investigated by automated patch clamp (Qpatch; Biolin Scientific, Sophion, Denmark) as previously described.19 Likewise, the effect on rNaV1.5 was evaluated as earlier described20 but with a modified voltage protocol so that rNaV1.5 currents were elicited with 1-second interval by a 50-ms depolarizing step to −20 mV from a holding potential of −120 mV (a total of 80 pulses).

Article Title: Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.
Article Snippet: Many spider-venom peptides are known to modulate the activity of the voltage-gated sodium (NaV) subtype 1.7 (NaV1.7) channel, which has emerged as a promising analgesic target.. In particular, a class of spider-venom peptides (NaSpTx1) has been found to potently inhibit NaV1.7 (nanomolar IC50), and has been shown to produce analgesic effects in animals.. However, one member of this family [m-TRTX-Hhn2b (Hhn2b)] does not inhibit mammalian NaV channels expressed in dorsal root ganglia at concentrations up to 100 mM.

Article Title: Reply to: How Many SARS-CoV-2 "Viroporins" Are Really Ion Channels?
Article Snippet: Using an automated patch clamp technique with gigaseal formation and Rs compensation (QPatch, Sophion, Denmark), we have in preliminary experiments compared induced vs. non-induced HEK293 cells stably expressing ORF10 under the control of tetracycline.



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Na v channel compound profile assessed on the Qube or Qpatch*. Ten-point titrations in the Qube automated patch-clamp assay determined MSD199 to be a potent and selective compound (IC 50 = 4.7 ± 0.8 nM, mean ± SD). MSD199 potency was too weak to calculate for all other Na v isoforms except Na v 1.4, which was 1781-fold shifted to 8370 nM mean IC 50 ± 1429.

Journal: Pain

Article Title: Analgesia and peripheral c-fiber modulation by selective Na v 1.8 inhibition in rhesus

doi: 10.1097/j.pain.0000000000003404

Figure Lengend Snippet: Na v channel compound profile assessed on the Qube or Qpatch*. Ten-point titrations in the Qube automated patch-clamp assay determined MSD199 to be a potent and selective compound (IC 50 = 4.7 ± 0.8 nM, mean ± SD). MSD199 potency was too weak to calculate for all other Na v isoforms except Na v 1.4, which was 1781-fold shifted to 8370 nM mean IC 50 ± 1429.

Article Snippet: Qube voltage-clamp experiments were performed using human embryonic kidney (HEK) 293 cell lines or Chinese Hamster Ovary cell lines stably expressing either human Na v 1.1, Na v 1.2, Na v 1.3, Na v 1.4, Na v 1.5, Na v 1.6, Na v 1.7, Na v 1.8, or Na v 1.9 on the Qube or Qpatch automated patch-clamp platforms (Sophion Bioscience A/S, Ballerup, Denmark).

Techniques: Patch Clamp